Use disposable plastic syringes and safety straight needles or a safety-winged blood collection set. Also, never leave a tourniquet on the arm for more than one minute without releasing it. The comprehensive metabolic panel (CMP) usually includes 14 tests the 8 from the BMP as well as 2 protein tests ( albumin and total protein) and 4 liver tests ( ALP, ALT, AST, and bilirubin ). 0000908091 00000 n Reneke J, Etzell J, Leslie S, Ng VL, Gottfried EL. What level of BNP indicates heart failure? 0000353809 00000 n Also, the gel barrier may not be intact and could cause improper separation of serum and cells, possibly affecting test results. Prepare the film immediately, as soon as the drop of blood has been placed on the slide. Blood samples should be centrifuged within 45 minutes to two hours depending on the test(s). Note: If the blood has to be mixed with an additive (gently invert the tube 4 to 10 times depending on the specimen tube being used), this must be done immediately after collection. Valli N, Georges A, Corcuff JB, et al. 0000622018 00000 n Do not use gel-barrier tubes to submit specimens for therapeutic drug monitoring. Serum Preparation From Red-top Tube. It is recommended to ask all patients who may be indicated for this test about biotin supplementation. The Innovance D-dimer assay is intended for use in conjunction with a nonhigh clinical pretest probability (PTP) assessment model to exclude deep vein thrombosis (DVT) and pulmonary embolism (PE).6 This test can be used to exclude VTE with nonhigh pretest probability (ie, low or low/moderate pretest probability). WebLavender-top (EDTA) tube Collection Do not collect in glass. Do not disturb the cell layer or carry any cells over into the pipette. 2021 Laboratory Corporation of America Holdings and Lexi-Comp Inc. All Rights Reserved. It measures the levels of a certain type of hormone in your blood called a B-type natriuretic peptide. Presence of one or more of the following may be indication for further investigation: hemoglobin <10 g/dL, hemoglobin >18 g/dL, MCV >100 fL, MCV <80 fL, MCHC >37%, WBC >20,000/mm3, WBC <2000/mm3, presence of sickle cells, spherocytes, Pappenheimer bodies, basophilic stippling, stomatocytes, schistocytes (fragmented RBCs), target cells, oval macrocytes, teardrop red blood cells, abnormal cell populations, nucleated red blood cells in other than the newborn, blood parasites (malarial or Babesia organisms or the possibility of parasitic organisms), hypersegmented neutrophils, agranular neutrophils, hyposegmented neutrophils (Pelger-Hut anomaly or pseudo-Pelger-Hut [pelgeroid] cells), mononuclear cells in which apparent nucleoli are prominent (blast-like cells), presence of metamyelocytes, myelocytes, promyelocytes, neutropenia, presence of plasma cells, peculiar atypical lymphocytes, significant increase or decrease in platelets or bizarre platelets. High levels of BNP or NT-proBNP are a sign that the heart has to work too hard to pump blood. In most cases, lab test results delivery times should not exceed two weeks. At the time of specimen pickup, inform your professional service representative that you have a frozen specimen to be picked up. To allow adequate mixing, slowly invert the tube eight to ten times (four times for citrate tubes) using a gentle wrist rotation motion. Turnaround time is defined as the usual number of days from the date of pickup of a specimen for Select two clean, grease-free glass collection slides with frosted ends (new ones whenever possible). Your levels can also be lower if you have obesity or kidney failure. Label tubes in front of the patient immediately after collection, confirming all necessary information with the patient. Turnaround time is defined as the usual number of days from the date of pickup of a specimen for 0000876850 00000 n Do not recap, cut, or bend any needles; dispose of them in a sharps container. The basic metabolic panel (BMP) may be used to check the health of your kidneys, the status of your electrolyte and acid/base balance, as well as your blood glucose level all of which are related to your bodys metabolism. In a healthy person, the white cells respond to minor infections by increasing in number and eliminating pathogens. 0000878985 00000 n Materials for proper specimen collection and transport are supplied by LabCorp. Air drying techniques are usually not satisfactory. trailer <]/Prev 982724>> startxref 0 %%EOF 77 0 obj <>stream Gel-barrier (mottled red/gray, gold, or cherry red-top) tubes contain clot activator and gel for separating serum from cells but include no anticoagulant. With some needle assemblies, you may slide the collection tube into the holder, carefully pushing the tubes forward until the needle touches the stopper. 0.000.49 mg/L FEU (Fibrinogen equivalent units), Coagulation activation results in the cleavage of fibrinogen to fibrin monomer.7,8 The fibrin monomers spontaneously aggregate to fibrin and are cross-linked by factor XIII; this produces a fibrin clot. Dao Q, Krishnaswamy P, Kazanegra R, et al. Note: Please examine specimen collection and transportation supplies to be sure they do not include expired containers. Occasionally, vacuum tubes lose their vacuum. Print the patient's name and date on the frosted ends of both slides. HtVK7)t(q "&}hDR}FFau6s}V'YQJw:,}_D&&@Ze@$:(!>!K\ir}~HU7;mBfU;eQ=t:xr2+^UVQUD9 #JZ!$g}! @/y}GLPSsh@'lK+G\1.JciuA>"j>_R9TDwGgnU:zP%4m0#HLGSN-,"\4,LD`UD+w=FFF%)K.a"8>IQv trXIz-Xd `Z#"T'.BdYY+'b*tm %u`B Wd?doNnehj=s}+ O!"Q^/3r4uH*{| y #[OdHrpFW\)rN#iQq|p_>&cFd!1~hXB$?=bxup XW6U@og4nKq%dhlK c@dc"5gCucFOHUKf*1q;Hh@#/[ \Rgc=Xk_{:s(. Small amounts of moisture can cause hemolysis. 0000005011 00000 n Analytical and clinical evaluation of the Bayer ADVIA Centaur Automated B-type natriuretic peptide assay in patients with heart failure: A multisite study. WebBlood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. In some cases, additional time should be Gel-barrier Tubes. To avoid clotting, mix the blood with the anticoagulant or preservative immediately after drawing each sample. testing to when the result is released to the ordering provider. If the glass syringe has been autoclaved, it should be oven dried before use. biotin therapy for Multiple Sclerosis or oncology patients; skin, hair and nail supplements, or multivitamins containing > 5 mg/day) until at least 12 hours after the last biotin administration. D-dimer levels can be elevated in many clinical circumstances, especially in hospitalized patients. Be sure to use the centrifuge that LabCorp has provided for your use in these separations. Maisel A. B-type natriuretic peptide levels: A potential novel white count for congestive heart failure. Yellow-top tube: Contains acid citrate dextrose (ACD) solution. 0000459642 00000 n A BNP test is done by drawing blood from a vein in your arm using a hypodermic needle. If you already know you have heart failure, your provider may order this test to check the severity (seriousness) of your condition. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. The left ventricle is one of the main organs that make BNP. Additional information regarding LOINC codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf. Maisel and coworkers4 found that a BNP cutoff of 75 pg/mL could be used to predict systolic and/or diastolic abnormalities accurately in symptomatic patients referred to echocardiology for left ventricular function studies. Age-adjusted D-dimer cutoff levels to rule out pulmonary embolism: The ADJUST-PE study. Place a piece of dry gauze over the needle and withdraw the needle carefully. Also, print on the label the type of plasma submitted (eg, Plasma, Sodium Citrate, Plasma, EDTA, etc). When multiple specimens are required, remove the first collection tube from the holder as soon as blood flow ceases, invert the first tube to prevent clotting, and gently insert the second tube into the holder. Do not remove the stopper. BLOOD COLLECTION GUIDE LABORATORY They found that a BNP value >80 pg/mL could be used to identify patients with CHF from this population with a sensitivity of 97%, specificity of 98%, positive predictive value of 92%, and a negative predictive value of 98%.6. Hemolysis can be caused by rough handling of a blood specimen, leaving the tourniquet on too long (causing blood stasis) or squeezing the tip of the finger too hard during capillary collection, dilution, exposure to contaminants, extremes in temperature, or pathologic conditions. As late as possible before the lockbox is to be put out, place the frozen transport tube in the Frozen Specimen Keeper between the silver frozen gel packs. 6. The drop of blood should be in the center line, approximately inch from the frosted end. 2021 Laboratory Corporation of America Holdings and Lexi-Comp Inc. All Rights Reserved. Note: When multiple specimens are drawn from a single venipuncture, the following order is recommended: (1) sterile blood culture tubes, (2) nonadditive clotting tubes (red), (3) coagulation tubes and tubes containing citrate (blue), (4) gel-barrier tubes and tubes with additives (red), (5) tubes containing heparin (green), (6) tubes containing EDTA (lavender, royal blue), (7) tubes containing acid citrate dextrose (yellow), and (8) tubes containing sodium fluoride and potassium oxalate (gray).
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